The Genome of Pasteuria ramosa

[Genome Project #61517]

Summary - Pasteuria penetrans Genome Project
Project Members :

Dr. Jean-Claude Walser (Genetic Diversity Centre, ETH Zurich)
Prof. Dr. Dieter Ebert (University of Basel)
Marjut Paljakka (University of Basel)
Sebastian Gygli (Swiss Tropical and Public Health Institute)

Introduction :

The genus Pasteuria consists of Gram-positive, endospore-forming bacteria, which are obligate parasites of invertebrates. Hosts include plant parasitic nematodes and planktonic freshwater crustaceans of the genus Daphnia (De Vos et al. 2009). Pasteuria spp. are related to members of the Bacillus/Clostridium clade, although their exact phylogenetic position is subject of debate (Charles et al. 2005). We are studying Pasteuria. ramosa, an obligate, extracellular parasite of Daphnia magna and other cladocera. It is extremely virulent and infects the host hemolymph, leading to chronic infections which cause host castration, gigantism and premature death. Pasteuria ramosa is transmitted strictly horizontal, whereby the endospores are released into the environment upon decay of deceased hosts (Ebert 2005). Attempts to understand the molecular diversity and genetic architecture of P. ramosa, however, have been hampered by the diffculty in extracting high quality genomic DNA from the extremely degradation resistant endospores.

News :

> Ferbruary 23, 2015 - New genome assembly based on Illumina MiSeq 600-cycle reads.

> Draft Genome Project Archive (will not be continued)

Assembly Summary :
Assembly
Assembly 150229
Assembly 150228
Clone (strain)
C1
C1
Data Set
MiSeq Run01 (150223)
MiSeq Run01 (150223)
 
Data Subset
Contigs > 10kb
Contigs > 10kb
Number of contigs
21
30
Total size
1,684,007 bp
1,682,852 bp
Avergae read coverage
471
96
   
Shortest contig
14,841 bp
15,461 bp
Longest contig
221,477 bp
191,712 bp
Average GC% content
29%
30%
Pictures :
D. magna female infected with P. ramosa. [courtesy of Duneau et al. 2011]
A spore of P. ramosa. [courtesy of Duneau et al. 2011]
Activted spores of P. ramosa. [courtesy of Duneau et al. 2011]